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The Biological Scientist II will perform research in the laboratory of Dr. Barry Byrne, MD, PhD in the Department of Pediatrics - Child Health Research Institute (CHRI).
Duties will include:
Animal Studies:
Handling mice for setting up timed matings, weaning animals and maintaining rodent colonies. Will
participate in treating animals with pharmacological agents or AAV vectors either through i.p., i.m., or i.v.
injection. Other duties with the mice may include doing echocardiography, sensory and motor function,
force mechanics, and metabolic studies. Collecting organs and other tissue and embedding the specimens
for sectioning or for molecular analyses.
Molecular techniques and analysis:
Molecular cloning, Plasmid purification, Primer design, RNA and DNA isolation, isolating RNA and
performing real-time PCR, and PCR genotyping of transgenic mouse lines. Participate in in vitro studies
exploring novel vectors in various cells. This may include expansion/maintenance of cell lines and analysis
of transfection/infection. Performing protein analysis by isolating protein of interest from cell or tissue
lysates and performing/analyzing western blots.
Project Management and Data Analysis:
Coordinate the ordering of supplies in advance of experimental need, assist in supervising or directing the
work of laboratory personnel, assist in teaching or advising work of laboratory personnel in laboratory
methods and/or procedures, assist in directing/coordinating/organizing laboratory activities; upholding
laboratory safety policy compliance of all members working in the laboratory, assist with regulatory
compliance (EHS, IACUC, etc). Compiling experimental data for presentation in various formats
(GraphPad, Excel, etc).
Performing various tasks as assigned.
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